A Novel DNA Biosensor Based on a Pencil Graphite Electrode Modified with Polypyrrole/Functionalized Multiwalled Carbon Nanotubes for Determination of 6-Mercaptopurine Anticancer Drug

作者：Karimi-Maleh, Hassan^*; Tahernejad-Javazmi, Fahimeh; Atar, Necip; Lutfi, Mehmet; Gupta, Vinod Kumar; Ensafi, Ali A.

来源：Industrial & Engineering Chemistry Research, 2015, 54(14): 3634-3639.

DOI：10.1021/ie504438z

• SENSITIVE VOLTAMMETRIC SENSOR
• PASTE ELECTRODE
• NANOPARTICLES
• NANOCOMPOSITE
• GLUTATHIONE
• QUERCETIN

A novel and sensitive biosensor employing immobilized DNA on a pencil graphite electrode modified with polypyrrole/functionalized multiwalled carbon nanotubes for the determination of 6-mercaptopurine (6-MP) is presented. In the first step, we modified the pencil graphite surface with polypyrrole and functionalized multiwalled carbon nanotubes (MWCNT/COOH). The developed electrode was characterized by scanning electron microscopy, atomic force microscopy, reflectionabsorption infrared spectroscopy, X-ray photoelectron spectroscopy, and electrochemical impedance spectroscopy. In the other step, we used decreases in the oxidation responses of guanine and adenine as a sign of the interaction of 6-MP with salmon sperm double-stranded DNA using differential pulse voltammetry. The signal of guanine oxidation was linear with respect to the 6-MP concentration in the range of 0.2-100 mu mol L-1 with a detection limit of 0.08 mu mol L-1. The modified electrode was utilized for the determination of 6-MP in real samples.

• 出版日期2015-4-15
• 单位中国石油大学（北京）