Developmental changes of the protein repertoire in the rat auditory brainstem: A comparative proteomics approach in the superior olivary complex and the inferior colliculus with DIGE and iTRAQ

作者：Kaltwasser Bernd; Schulenborg Thomas; Beck Florian; Klotz Markus; Schaefer Karl Herbert; Schmitt Melanie; Sickmann Albert; Friauf Eckhard^*

来源：Journal of Proteomics, 2013, 79: 43-59.

DOI：10.1016/j.jprot.2012.11.018

• 2-D DIGE
• Inferior colliculus
• iTRAQ
• Metabolic enzymes
• Stathmin-1
• Superior olivary complex

Protein profiles of developing neural circuits undergo manifold changes. The aim of this proteomic analysis was to quantify postnatal changes in two auditory brainstem areas in a comparative approach.. Protein samples from the inferior colliculus (IC) and the superior olivary complex (SOC) were obtained from neonatal (P4) and young adult (P60) rats. The cytosolic fractions of both areas were examined by 2-D DIGS, and the plasma membrane-enriched fraction of the IC was analyzed via iTRAQ. iTRAQ showed a regulation in 34% of the quantified proteins. DIGS revealed 12% regulated spots in both the SOC and IC and, thus, numeric congruency. Although regulation in KEGG pathways displayed a similar pattern in both areas, only 13 of 71 regulated DIGE proteins were regulated in common, implying major area-specific differences. 89% of regulated glycolysis/gluconeogenesis and citrate cycle proteins were up-regulated in the SOC or IC, suggestitig a higher energy demand in adulthood. Seventeen cytoskeleton proteins were regulated, consistent with complex morphological reorganization between P4 and P60. Fourteen were uniquely regulated in the SOC, providing further evidence for area-specific differences. Altogether, we provide the first elaborate catalog of proteins involved in auditory brainstem development, several of them possibly of particular developmental relevance.

• 出版日期2013-2-21