Antifeedant activity of ethanolic extract from Flourensia oolepis and isolation of pinocembrin as its active principle compound

作者：Diaz Napal Georgina N; Carpinella Maria C; Palacios Sara M^*

来源：Bioresource Technology, 2009, 100(14): 3669-3673.

DOI：10.1016/j.biortech.2009.02.050

• Flourensia oolepis
• Asteraceae
• Antifeedant
• Flavonoids
• Pinacembrin

The ethanolic extract from Flourensia oolepis aerial parts showed strong antifeedant activity against the pest larvae, Epilachna paenulata, with an antifeedant index (Al%) of 99.1% at 100 mu g/cm(2). Based on chromatographic fractionation of the extract, guided by bioassays on E. paenulata, the flavanone pinocembrin (1) was isolated as the most active principle. In a choice assay, compound I showed strong antifeedant activity against E. paenulata, Xanthogaleruca luteola and Spodoptera frugiperda with an Al% of 90, 94 and 91% (p < 0.01) respectively, at 50 mu g/cm(2). The dosages necessary for 50% feeding inhibition of the insects (ED50) were 7.98, 6.13 and 8.86 mu g/cm(2), respectively. The feeding inhibitory activity of 1 against E. paenulata was compared with the activity of other structurally related flavonoids like naringenin, which was inactive up to 100 mu g/cm(2), catechin which was nearly 6 times less active than 1, and quercetin which was equally active as 1. The effect of these on the feeding behavior of E. paenulata was also studied.

• 出版日期2009-7