Aggregate formation in tauroursodeoxycholate solutions

作者：Liu Chen Lun^*; Kankala Ranjith K; Yang June Wen; Hsu You Chun; Lee Chia Hung; Hu Yu Fang

来源：Journal of the Taiwan Institute of Chemical Engineers, 2014, 45(4): 1285-1290.

DOI：10.1016/j.jtice.2013.12.006

• Hydrodynamic radius
• Laser light-scattering
• Kinetic dialysis technique

The hydrodynamic radius (r(H)) values of simple tauroursodeoxycholate (TUDC) micelles formed between 12 mM and 60 mM TUDC solutions were measured using a laser light-scattering method to be nearly constant (similar to 15.4 angstrom). Additionally, a kinetic dialysis technique was employed to determine the TUDC monomer concentrations with respect to the total TUDC concentrations. According to the chemical equilibrium treatment, a relationship between the TUDC monomer concentrations and the total TUDC concentrations was derived. By using a least-squares technique, the best curve-fitting to the kinetic dialysis data of the relationship predicted by the mass-action model was obtained. The equilibrium constants were also quantified. Their values together with the light-scattering results indicate that both TUDC dimers and simple TUDC micelles are formed from TUDC monomers by hydrophobic interaction. Moreover, the concentration of TUDC dimers is much higher than the concentration of simple TUDC micelles in TUDC solutions.

• 出版日期2014-7
• 单位东华大学