Gene Expression Enabling Synthetic Diversification of Natural Products: Chemogenetic Generation of Pacidamycin Analogs

作者：Roy Abhijeet Deb; Grueschow Sabine; Cairns Nickiwe; Goss Rebecca J M^*

来源：Journal of the American Chemical Society, 2010, 132(35): 12243-12245.

DOI：10.1021/ja1060406

Introduction of prnA, the halogenase gene from pyrrolnitrin biosynthesis, into Streptomyces coeruleorubidus resulted in efficient in situ chlorination of the uridyl peptide antibotic pacidamycin. The installed chlorine provided a selectably functionalizable handle enabling synthetic modification of the natural product using mild cross-coupling conditions in crude aqueous extracts of the culture broth.

• 出版日期2010-9-8