Development of a non-destructive method for determining protein nitrogen in a yellow fever vaccine by near infrared spectroscopy and multivariate calibration

作者：Dabkiewicz Vanessa Emidio^*; Pereira Abrantes Shirley de Mello; Cassella Ricardo Jorgensen

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2018, 201: 170-177.

DOI：10.1016/j.saa.2018.04.042

• Nitrogen
• Protein
• Near infrared
• Multivariate calibration
• Kjeldahl
• Cluster analysis
• Mahalanobis distance

Near infrared spectroscopy (NIR) with diffuse reflectance associated to multivariate calibration has as main advantage the replacement of the physical separation of interferents by the mathematical separation of their signals, rapidly with no need for reagent consumption, chemical waste production or sample manipulation. Seeking to optimize quality control analyses, this spectroscopic analytical method was shown to be a viable alternative to the dassical Kjeldahl method for the determination of protein nitrogen in yellow fever vaccine. The most suitable multivariate calibration was achieved by the partial least squares method (PLS) with multiplicative signal correction (MSC) treatment and data mean centering (MC), using a minimum number of latent variables (LV) equal to 1, with the lower value of the square root of the mean squared prediction error (0.00330) associated with the highest percentage value (91%) of samples. Accuracy ranged 95 to 105% recovery in the 4000-5184 cm(-1) region.

• 出版日期2018-8-5