A novel glucose biosensor based on direct electrochemistry of glucose oxidase incorporated in biomediated gold nanoparticles-carbon nanotubes composite film

作者：Zhang Hongfang; Meng Zuchao; Wang Qi; Zheng Jianbin^*

来源：Sensors and Actuators B: Chemical , 2011, 158(1): 23-27.

DOI：10.1016/j.snb.2011.04.057

• Direct electrochemistry
• Enzymatic reaction
• Gold nanoparticles
• Glucose oxidase
• Glucose

A novel glucose biosensor based on biomediated gold nanoparticles (AuNPs)-glucose oxidase (GOD)-carbon nanotubes-polyvinyl alcohol biocomposite film was fabricated for the first time. Direct electrochemistry of GOD in the film was obtained, proved by one pair of well-defined, quasi-reversible redox peaks with the formal potential of -0.504V in pH 7.0 phosphate buffer solution at the scan rate of 100 mV s(-1). And the electron transfer rate constant of the direct electron communication between GOD and the electrode was calculated to be 2.2 s(-1). The biomediated AuNPs play an important role in facilitating the electron exchange between the electroactive center of GOD and the electrode. Furthermore, the fabricated electrode displayed a linear amperometric response in the glucose concentration range from 0.5 to 8.0 mM with a relatively high sensitivity of 16.6 mu A mM(-1) cm(-2). Therefore, the AuNPs based biosensor described here provides a different mode to design electrochemical devices.

• 出版日期2011-11-15
• 单位西北大学