科研之友
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摘要
Sialylated glycans are crucial molecular targets for cancer diagnosis and clinical research. alpha 2,3-Sialylated glycans and alpha 2,6-sialylated glycans are the predominant sialic acids found in nature. Different expression of the quantity of glycans can result in development of different disease. However, there are no ideal methods for discriminating alpha 2,3-sialylated glycans and alpha 2,6-sialylated glycans. In this work, a multi-purpose biosensor is fabricated for sensitive detection of alpha 2,3-sialylated glycans and alpha 2,6-sialylated glycans. To improve the sensitivity of the biosensor, p-MWCNTs were integrated with PAMAM, as PAMAM has highly branched and abundant amino groups, providing a large available surface area for linking with other substances. To achieve distinguishable recognition, Maackia amurensis lectin (MAL) and Sambucus nigra agglutinin (SNA) were included. To facilitate the lectin fixation, PDITC, a kind of green homobifunctional cross-linker, was selected. Under optimized detection conditions, the linear range of detection for alpha 2,3-sialylated glycans is 10 fg mL(-1) to 50 ng mL(-1) with a lower detection limit of 3 fg mL(-1), and the linear range of detection for alpha 2,6-sialylated glycans is 10 fg mL(-1) to 50 ng mL(-1) with a detection limit of 3 fg mL(-1). This work not only provides a method for distinguishing detection of alpha 2,3-sialylated glycans and alpha 2,6-sialylated glycans, but also provides a reference for future clinical testing.
- 出版日期2016
- 单位重庆医科大学
