科研之友
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摘要
The aim of the present investigation was to examine and demonstrate in detail the anti -cancer and apoptotic effects of daucosterol in human colon cancer cell line HCT-116. The effects of this compound on cell migration, cell invasion, cell cycle analysis and caspase signalling pathway were also studied. Cell viability was evaluated by MTT assay using different doses of the drug. In vitro wound healing assay was used to study cell migration. Flow 9rtometry was involved to examine cell apoptosis as well as cell cycle phase distribution. Daucosterol induced significant, dose -dependent as well as time dependent cytotoxic effects with ICso values of 26.6 and 47.3 M at 24 and 48 hours time intervals respectively. The percent of cells that migrated decreased from 99% in case of untreated control to 84.2, 45.2, 39.5 and 14.4', in groups treated with 0, 5, 50, 75 and 100 gM of daucosterol respedively. Percentage of apoptotic cells increased frorn 2.5% in untreated control cells to 23.6, 46.9 and 74.2% in cells treated with 5, 50 and 100 11M dose of daucosterol respectively. lDaucosterol at different doses induced cell cycle arrest at sub -G1 phase of the cell cycle.
- 出版日期2016
- 单位河北医科大学
