Arcanobacterium pinnipediorum sp nov., isolated from a harbour seal

作者：Sammra Osama; Balbutskaya Anna; Uelbegi Mohyla Hivda; Nagib Samy; Laemmler Christoph; Kaempfer Peter; Glaeser Stefanie P; Golke Jan; Busse Hans Juergen; Prenger Berninghoff Ellen; Siebert Ursula; Abdulmawjood Amir; Klein Guenter

来源：International Journal of Systematic and Evolutionary Microbiology, 2015, 65(12): 4539-4543.

DOI：10.1099/ijsem.0.000609

A polyphasic taxonomic study was performed on an unidentified Arcanobacterium-like, Gram-stain-positive bacterium, strain 2710(T), isolated from a harbour seal. Comparative 16S rRNA gene sequence analysis showed that this bacterial strain belonged to the genus Arcanobacterium and was related most closely to the type strains of Arcanobacterium phocae (98.4 % similarity) and Arcanobacterium phocisimile (97.5 %). 16S rRNA gene sequence similarities to the type strains of other Arcanobacterium species were between 95.3 and 96.9 0/o. DNA-DNA hybridization values between strain 2710(T) and A. phocae DSM 10002(T) and A. phocisimile LMG 27073(T) were 4.7 % (reciprocal 56 %) and 23 % (reciprocal 7.7 %), respectively. The presence of the major menaquinone MK-9(H4) and a polar lipid profile with the major compounds diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside supported the affiliation of strain 2710(T) to the genus Arcanobacterium. The major fatty acids were C16:0, C18:1 omega 9c, C18:0 and C18:2 omega 6,9c/anteiso-C18:0. The peptidoglycan structure was of cross-linkage type A5 alpha (L-Lys L-Lys D-Glu). Physiological and biochemical tests clearly distinguished the isolate from other members of the genus Arcanobacterium. Based on these tests, it is proposed that this unknown bacterium should be classified as a novel species of the genus Arcanobacterium, with the name Arcanobacterium pinnipediorum sp. nov. The type strain is 2710(T) (=DSM 28752(T)=LMG 28298(T)).

• 出版日期2015-12