A multichannel nanosensor for instantaneous readout of cancer drug mechanisms

作者：Rana Subinoy^*; Le Ngoc D B; Mout Rubul; Saha Krishnendu; Tonga Gulen Yesilbag; Bain Robert E S; Miranda Oscar R; Rotello Caren M; Rotello Vincent M

来源：Nature Nanotechnology, 2015, 10(1): 65-69.

DOI：10.1038/NNANO.2014.285

Screening methods that use traditional genomic(1-3), transcriptional(4), proteomic(5,6) and metabonomic(7) signatures to characterize drug mechanisms are known. However, they are time consuming and require specialized equipment. Here, we present a high-throughput multichannel sensor platform that can profile the mechanisms of various chemotherapeutic drugs in minutes. The sensor consists of a gold nanoparticle complexed with three different fluorescent proteins that can sense drug-induced physicochemical changes on cell surfaces(8-10). In the presence of cells, fluorescent proteins are rapidly displaced from the gold nanoparticle surface and fluorescence is restored. Fluorescence 'turn on' of the fluorescent proteins depends on the drug-induced cell surface changes, generating patterns that identify specific mechanisms of cell death induced by drugs. The nanosensor is generalizable to different cell types and does not require processing steps before analysis, offering an effective way to expedite research in drug discovery, toxicology and cell-based sensing.

• 出版日期2015-1