ALVEOLAR MACROPHAGE PHAGOCYTOSIS IS ENHANCED AFTER BLUNT CHEST TRAUMA AND ALTERS THE POSTTRAUMATIC MEDIATOR RELEASE

作者:Seitz Daniel H*; Palmer Annette; Niesler Ulrike; Froeba Janine S; Heidemann Vera; Rittlinger Anne; Braumueller Sonja T; Zhou Shaoxia; Gebhard Florian; Knoeferl Markus W
来源:Shock, 2011, 36(6): 621-627.
DOI:10.1097/SHK.0b013e318234f8a0

摘要

Blunt chest trauma is known to induce a pulmonary invasion of short-lived polymorphonuclear neutrophils and apoptosis of alveolar epithelial type 2 (AT2) cells. Apoptotic cells are removed by alveolar macrophages (AM Phi). We hypothesized that chest trauma alters the phagocytic response of AM Phi as well as the mediator release of AM Phi during phagocytosis. To study this, male Sprague-Dawley rats were subjected to blunt chest trauma. Phagocytosis assays were performed in AM Phi isolated 2 or 24 h after trauma with apoptotic cells or opsonized beads. Phagocytosis of apoptotic AT2 cells by unstimulated AM Phi was significantly increased 2 h after trauma. At 24 h, AM Phi from traumatized animals, stimulated with phorbol-12-myristate-13-acetate, ingested significantly more apoptotic polymorphonuclear neutrophils than AM Phi from sham animals. Alveolar macrophages after trauma released significantly higher levels of tumor necrosis factor alpha, macrophage inflammatory protein 1 alpha, and cytokine-induced neutrophil chemoattractant 1 when they incorporated latex beads, but significantly lower levels of interleukin 1 beta and macrophage inflammatory protein 1 alpha when they ingested apoptotic cells. In vivo, phagocytosis of intratracheally instilled latex beads was decreased in traumatized rats. The bronchoalveolar lavage concentrations of the phagocytosis-supporting surfactant proteins A and D after blunt chest trauma were slightly decreased, whereas surfactant protein D mRNA expression in AT2 cells was significantly increased after 2 h. These findings indicate that chest trauma augments the phagocytosis of apoptotic cells by AM Phi. Phagocytosis of opsonized beads enhances and ingestion of apoptotic cells downregulates the immunologic response following lung contusion. Our data emphasize the important role of phagocytosis during posttraumatic inflammation after lung contusion.

  • 出版日期2011-12