Antibody-based analysis reveals %26quot;filamentous vs. non-filamentous%26quot; and %26quot;cytoplasmic vs. nuclear%26quot; crosstalk of cytoskeletal proteins

作者：Kumeta Masahiro^*; Hirai Yuya; Yoshimura Shige H; Horigome Tsuneyoshi; Takeyasu Kunio

来源：Experimental Cell Research, 2013, 319(20): 3226-3237.

DOI：10.1016/j.yexcr.2013.07.021

• Cytoskeleton
• Keratin
• Monoclonal antibody
• Nuclear matrix
• Nuclear scaffold
• Nuclear transport

To uncover the molecular composition and dynamics of the functional scaffold for the nucleus, three fractions of biochemically-stable nuclear protein complexes were extracted and used as immunogens to produce a variety of monoclonal antibodies. Many helix-based cytoskeletal proteins were identified as antigens, suggesting their dynamic contribution to nuclear architecture and function. Interestingly, sets of antibodies distinguished distinct subcellular localization of a single isoform of certain cytoskeletal proteins; distinct molecular forms of keratin and actinin were found in the nucleus. Their nuclear shuttling properties were verified by the apparent nuclear accumulations under inhibition of CRM1-dependent nuclear export Nuclear keratins do not take an obvious filamentous structure, as was revealed by non-filamentous cytoplasmic keratin-specific monoclonal antibody. These results suggest the distinct roles of the helix-based cytoskeletal proteins in the nucleus.

• 出版日期2013-12-10