Absence of Neuroplastin-65 Affects Synaptogenesis in Mouse Inner Hair Cells and Causes Profound Hearing Loss

作者：Carrott Leanne; Bowl Michael R^*; Aguilar Carlos; Johnson Stuart L; Chessum Lauren; West Melissa; Morse Susan; Dorning Joanne; Smart Elizabeth; Hardisty Hughes Rachel; Ball Greg; Parker Andrew; Barnard Alun R; MacLaren Robert E; Wells Sara; Marcotti Walter; Brown Steve D M

来源：Journal of Neuroscience, 2016, 36(1): 222-234.

DOI：10.1523/JNEUROSCI.1808-15.2016

• exocytosis
• hearing loss
• inner hair cell
• neuroplastin
• ribbon synapse
• synaptogenesis

The Neuroplastin gene encodes two synapse-enriched protein isoforms, Np55 and Np65, which are transmembrane glycoproteins that regulate several cellular processes, including the genesis, maintenance, and plasticity of synapses. We found that an absence of Np65 causes early-onset sensorineural hearing loss and prevented the normal synaptogenesis in inner hair cells (IHCs) in the newly identified mouse mutant pitch. In wild-type mice, Np65 is strongly upregulated in the cochlea from around postnatal day 12 (P12), which corresponds to the onset of hearing. Np65 was specifically localized at the presynaptic region of IHCs. We found that the colocalization of presynaptic IHC ribbons and postsynaptic afferent terminals is greatly reduced in pitch mutants. Moreover, IHC exocytosis is also reduced with mutant mice showing lower rates of vesicle release. Np65 appears to have a nonessential role in vision. We propose that Np65, by regulating IHC synaptogenesis, is critical for auditory function in mammals.

• 出版日期2016-1-6