科研之友
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摘要
Amphiphilic fluorescent graft copolymer (PVP-PyATAm) was successfully synthesized by the free radical copolymerizations of hydrophobic monomer N-acryloyl-thioureylene-4-(1-pyrene)-butyryl amide (PyATAm) with hydrophilic precursor polymers of vinyl-functionalized poly (N-vinylpyrrolidone) (Acryloyl-PVP) in DMF. FT-IR, H-1 NMR, TEM, gel permeation chromatography-multi-angle laser light scattering, UV-vis spectroscopy, viscometric measurement, and fluorescence spectroscopy were used to characterize this copolymer. The TEM observation showed that the copolymer PVP-PyATAm formed spherical micelles in an aqueous solution and the size of micelles was between 50 and 70 nm in diameter. The interaction of PVP-PyATAm copolymer and plasmid DNA was examined by agarose gel electrophoresis and TEM. Results indicated that the copolymer-DNA complexes were self-assembled and the size of complexes was between 90 and 120 nm in diameter. Cytotoxity studies using MTT colorimetric assays suggested good biocompatibility of PVP-PyATAm in vitro. These results suggested the potential of this graft copolymer as gene delivery carrier.
