Detection of porcine circovirus type 2, porcine parvovirus and porcine pseudorabies virus from pigs with postweaning multisystemic wasting syndrome by multiplex PCR

作者：Cao S; Chen H^*; Zhao J; Lu J; Xiao S; Jin M; Guo A; Wu B; He Q

来源：Veterinary Research Communications, 2005, 29(3): 263-269.

DOI：10.1023/B:VERC.0000047501.78615.0b

• multiplex PCR
• porcine circovirus type 2
• porcine parvovirus
• porcine pseudorabies virus
• postweaning multisystemic wasting syndrome

Multiplex PCR was established to detect porcine circovirus type 2 (PCV-2), porcine parvovirus (PPV) and porcine pseudorabies virus (PRV) and applied to samples from 137 piglets exhibiting clinical signs of postweaning multisystemic wasting syndrome (PMWS). PCV-2 DNA was detected from all samples. Moreover, 43 samples were positive for PPV but negative for PRV; 11 samples were positive for PRV but negative for PPV; and 35 samples were positive both for PPV and PRV. These results suggests that PCV-2 co-infection with PRV and PPV may play an important role in PMWS. Also, multiplex PCR is an appropriate candidate method for diagnosis of PCV-2, PRV and PPV simultaneously in field cases.

• 出版日期2005-4
• 单位华中农业大学