Anticancer potential of ZnO nanoparticle-ferulic acid conjugate on Huh-7 and HepG2 cells and diethyl nitrosamine induced hepatocellular cancer on Wistar albino rat

作者：Ezhuthupurakkal Preedia Babu; Ariraman Subastri; Arumugam Suyavaran; Subramaniyan Nithyananthan; KumarMuthuvel Suresh; Kumpati Premkumar; Rajamani Bharathidasan; Chinnasamy Thirunavukkarasu^*

来源：Nanomedicine: Nanotechnology, Biology and Medicine , 2018, 14(2): 415-428.

DOI：10.1016/j.nano.2017.11.003

• ZnO nanoparticles
• Ferulic acid
• ZnONPs-ferulic acid conjugate
• Hepatocellular cancer
• Diethylnitrosamine

Drawbacks and limitations of recently available therapies to hepatocellular cancer (HCC) devoted the scientist to focus on emerging new strategies. ZnO nanoparticles (ZnONPs) based chemotherapeutics has been emanating as a promising approach to maximize therapeutic synergy facilitating the discovery of novel multitargeted combinations. In the present study we conjugated ZnONPs with ferulic acid (ZnONPs-FAC) characterized by computational, spectroscopic and microscopic techniques. In vitro anticancer potential has been evaluated by assessing cell viability, morphology, ROS generation, mitochondrial membrane permeability, comet assay, immunofluorescent staining of 8-OHdG, Ki67 and gamma-H2AX, cell cycle analysis and western blot analysis and in vivo anticancer potential against DEN induced HCC was analyzed by histopathological and immunohisto chemical methods. The results revealed that ZnONPs-FAC induces cell death through apoptosis and can suppress the DEN-induced HCC. Our study documents therapeutic potential of nanoparticle conjugated with phytochemicals, suggesting a new platform for combinatorial chemotherapy.

• 出版日期2018-2
• 单位上海生物信息技术研究中心