Molecular phylogeny of Phalaenopsis Blume (Orchidaceae) on the basis of plastid and nuclear DNA

作者:Tsai C C; Chiang Y C; Huang S C; Chen C H; Chou C H*
来源:Plant Systematics and Evolution, 2010, 288(1-2): 77-98.
DOI:10.1007/s00606-010-0314-1

摘要

The Internal transcribed spacers (ITS) of nuclear ribosomal DNA (nrDNA) and plastid DNA, including the trnL intron, the trnL-F spacer, and the atpB-rbcL spacer, from most of the living species in the genus Phalaenopsts were sequenced. The monophyly of the genus described by Christenson (Christenson EA (2001) Phalaenopsis. Timber Press, Portland, p 330), that Doritis and Kingidium are synonyms of Phalaenopsis, was supported by these molecular data. Within the genus, subgenus Polychdos was monophyletic, and the species were divided Into two subclades. The subgenus Phalaenopsis was shown to be non-monophyletic, because the sections Esmeralda and Deltciosae appeared separated the from sections Pha Meows's and Stauroglottts Meanwhile, subgenera Aphyllae and Parishianae were also shown to be non-monophyletic on the basis of the molecular data. Furthermore, the monotypic species of subgenus Proboscitholdes, P. low'', formed a clade with subgenus Aphyllae In accordance with geographical distribution, the historical geography of Southeast Asia due to the periodic glacial epochs, and molecular phylogeny, two evolutionary trends of Phalaenopsis from the original center in South China to the Philippines, Indonesia, and Malaysia were suggested First, using Indochina and some older parts of the Philippines (e g, Mindoro and Palawan) as stepping stones, Phalaenopsis species dispersed from South China to the Philippines, where the sections Phalaenopsis and Staumglows of subgenus Phalaenopsis developed. Second, using the Malay Peninsula as a stepping stone, Phalaenopsis species dispersed from South China to Indonesia and Malaysia, where the subgenus Polychdos developed.