摘要

This paper mainly reports the alcohol-induced effection. Accoding to the mass spectrometry, we got a lot differences among myoglobin conformations induced by different alcohol at pH = 4.0, 30% (volume fraction) alcohol. The results show that alcohol's molecular structures effect Mb conformation. Besides, we found that the alkyl's number enhanced denaturation effection. Fluorine substituent could stabilize hMb and aMb selectively a certain extent. we also proved the regularity summarized as above farther and got more useful information of Fe ion's coordination and election spin state via CD spectroscopy and UV-Vis Soret Absorption.