Differences in Transport Mechanisms of trans-1-Amino-3-[F-18]Fluorocyclobutanecarboxylic Acid in Inflammation, Prostate Cancer, and Glioma Cells: Comparison with L-[Methyl-C-11]Methionine and 2-Deoxy-2-[F-18]Fluoro-D-Glucose

作者：Oka Shuntaro^*; Okudaira Hiroyuki; Ono Masahiro; Schuster David M; Goodman Mark M; Kawai Keiichi; Shirakami Yoshifumi

来源：Molecular Imaging and Biology, 2014, 16(3): 322-329.

DOI：10.1007/s11307-013-0693-0

• Anti-[F-18]FACBC
• Anti-[C-14]FACBC
• [C-11]Met
• [C-14]Met
• [F-18]FDG
• [C-14]FDG
• Amino acid transporters
• Inflammatory cells
• Prostate cancer
• Glioma

We aimed to elucidate trans-1-amino-3-[F-18]fluorocyclobutanecarboxylic acid (anti-[F-18]FACBC) uptake mechanisms in inflammatory and tumor cells, in comparison with those of l-[methyl-C-11]methionine ([C-11]Met) and 2-deoxy-2-[F-18]fluoro-d-glucose ([F-18]FDG). %26lt;br%26gt;Using carbon-14-labeled tracers, in vitro time-course, pH dependence, and competitive inhibition uptake experiments were performed in rat inflammatory (T cells, B cells, granulocytes, macrophages), prostate cancer (MLLB2), and glioma (C6) cells. %26lt;br%26gt;Anti-[C-14]FACBC uptake ratios of T/B cells to tumor cells were comparable, while those of granulocytes/macrophages to tumor cells were lower than those for [C-14]FDG. Over half of anti-[C-14]FACBC uptake by T/B and tumor cells was mediated by Na+-dependent amino acid transporters (system ASC), whereas most [C-14]Met transport in all cells was mediated by Na+-independent carriers (system L). %26lt;br%26gt;The low anti-[F-18]FACBC accumulation in granulocytes/macrophages may be advantageous in discriminating inflamed regions from tumors. The significant anti-[F-18]FACBC uptake in T/B cells may cause false-positives in some cancer patients who undergo FACBC-positron emission tomography (PET).

• 出版日期2014-6