Mitogenic Activity of CEL-I, an N-Acetylgalactosamine (GalNAc)-Specific C-Type Lectin, Isolated from the Marine Invertebrate Cucumaria echinata (Holothuroidea)

作者：Jiang Zedong; Kim Daekyung; Yamasaki Yasuhiro; Yamanishi Tomohiro; Hatakeyama Tomomitsu; Yamaguchi Kenichi; Oda Tatsuya^*

来源：Bioscience Biotechnology and Biochemistry, 2010, 74(8): 1613-1616.

DOI：10.1271/bbb.100207

• CEL-I
• Ca2+-dependent lectin (C-type lectin)
• Cucumaria echinata
• mitogenic activity

An N-acetylgalactosamine (GalNAc)-specific Ca(2+-)dependent lectin (C-type lectin), isolated from the marine invertebrate Holothuroidea (Cucumaria echinata), CEL-I, showed potent mitogenic activity toward normal mouse spleen cells. The mitogenic activity of CEL-I, which reached a maximum at 100 mu g/ml, was inhibited by GalNAc in a concentration-dependent manner. The mitogenic effect of CEL-I at 10 mu g/ml on T cell-enriched splenocytes was at a similar level due to a well-known T cell mitogen, concanavalin A (Con A), at 10 mu g/ml. Furthermore, CEL-I evoked a mitogenic response from nude mouse spleen cells, while no significant effects of Con A on this cell population were observed over a wide range of concentrations. These results suggest that CEL-I is a potent mitogenic lectin with the ability to stimulate both T and B cells.

• 出版日期2010-8