Aging of different avian cultured cells: Lack of ROS-induced damage and quality control mechanisms

作者：Strecker Valentina; Mai Soeren; Muster Britta; Beneke Sascha; Buerkle Alexander; Bereiter Hahn Juergen; Jendrach Marina^*

来源：Mechanisms of Ageing and Development, 2010, 131(1): 48-59.

DOI：10.1016/j.mad.2009.11.005

• Birds
• Aging
• ROS
• Autophagy
• ATG5
• Compensatory proliferation

Elevated reactive oxygen species (ROS) levels have been observed in mammals during aging, implying an important role of ROS in the aging process. Most bird species are known to live longer and to contain lower ROS levels than mammals of the same body weight. The influence of ROS on the aging process of birds has been investigated using pigeon embryonic fibroblasts (PEF) and chicken embryonic fibroblasts (CEF). ROS levels in young avian cells were much lower than in human cells. When cultivated till replicative senescence, PEF proliferated about one-third longer compared to CEF However, both senescent avian cell populations showed no increased ROS levels or accumulation of ROS-induced damage on the mtDNA or protein level. The investigation for quality control (QC) mechanisms revealed that the autophagosomal/lysosomal pathway was not downregulated in old avian cells and stable overexpression of the autophagy protein ATG5 improved mitochondrial fitness, enhanced the resistance against oxidative stress and prolonged the life span of CEF Oxidative stress-mediated apoptosis induced a dose-dependent cell proliferation in CEF as well as in PEF Taken togethe

• 出版日期2010-1