Activity of essential oils of Piper aduncum anf and Cinnamomum zeylanicum by evaluating osmotic and morphologic fragility of erythrocytes

作者：Barros Francisca Janielle; Oliveira Costa Raira Justino; Alves Santana Cesario Francisco Rafael; Rodrigues Lindaiane Bezerra; Martins da Costa Jose Galberto; Melo Coutinho Henrique Douglas; Figueiredo Galvao Hericka Bruna; Alencar de Menezes Irwin Rose

来源：European Journal of Integrative Medicine, 2016, 8(4): 505-512.

DOI：10.1016/j.eujim.2016.02.011

• Piper aduncum
• Cinnamomum zeylanicum
• Essential oil
• Morphology
• Red blood cells
• Osmotic fragility

Introduction: Free radicals generated in vivo and peroxidation of the unsaturated bonds of membrane lipids increases fragility and cellular lysis of red blood cells. The essential oils of Piper aduncum and Cinnamomum zeylanicum present antioxidant activity and are popular in some countries to treat gastrointestinal diseases and anxiolytic symptoms respectively. Osmotic fragility assays and morphometric analyses have been used to assess cytotoxic effects and to verify the interaction of compounds present in the essential oils using the membranes of red blood cells (RBC). The aim of this work was to evaluate the effects of the essential oils of P. aduncum and C. zeylanicum on osmotic fragility using osmotic stress and morphology of RBC.
Methodology: The essential oils were analysed by GC-MS. Blood samples were treated with essential oils in different concentrations; osmotic fragility was determined using saline stress assays and morphological analyses were carried out using light microscopy.
Results: In the presence of the oils, the data obtained indicated (i) a significant increase in hemolysis and (ii) modifications to the morphology of RBC with consequent structural modifications, increasing the permeability and diffusion of essential oils and provoking toxic effects over the red blood cells membranes.
Conclusion: In conclusion, the presence of essential oils from the leaves from P. aduncum and the bark of C. zeylanicum, significantly increased the osmotic fragility curve of red blood cells, and inducing toxic effects on red blood cell membranes.

• 出版日期2016-8