Are we ready to detect nematode diversity by next generation sequencing?

作者：Peham Thomas; Steiner Florian M; Schlick Steiner Birgit C; Arthofer Wolfgang

来源：Ecology and Evolution, 2017, 7(12): 4147-4151.

DOI：10.1002/ece3.2998

<jats:title>Abstract</jats:title><jats:p>In a Technical Advance article, Porazinska et al. (2009, Molecular Ecology Resources, 9, 1439–1450) assessed next generation sequencing (<jats:styled-content style="fixed-case">NGS</jats:styled-content>) as a method for metagenomic analysis of nematode diversity. We agree that <jats:styled-content style="fixed-case">NGS</jats:styled-content> has great potential here. However, it is not an easy path to the successful implementation of <jats:styled-content style="fixed-case">NGS</jats:styled-content> for environmental <jats:styled-content style="fixed-case">DNA</jats:styled-content> analysis of nematodes. Here, we describe the method's limitations and discuss prospective research questions. For instance, only a few direct extraction kits are suitable for nematode <jats:styled-content style="fixed-case">DNA</jats:styled-content> extraction from bulk samples without adaptation. They enable the analysis of extracellular nematode <jats:styled-content style="fixed-case">DNA</jats:styled-content>. The most crucial and unresolved issue remains the limited availability of suitable primers.</jats:p>

• 出版日期2017-6