Application of Campylobacter molecular classification and typing techniques in veterinary medicine: old-established methods and new perspectives

作者：Laturnus Claudia; Wieler Lothar H^*

来源：Berliner und Munchener Tierarztliche Wochenschrift, 2007, 120(7-8): 267-278.

DOI：10.2376/0005-9366-120-267

• Campylobacter spp.
• molecular epidemiology
• genotypical classification
• genotypical typing
• whole genome analysis
• single locus analysis
• multiple locus analysis
• surveillance
• monitoring

In this review the application and usefulness of Campylobacter genotypical classification and typing in veterinary medicine will be discussed. While there is a large area of overlapping applications between the veterinary and the medical field, several differences exist, as the spectrum of veterinary pathogens is different from the human and contaminated food of healthy animal origin may cause disease in man.
In general, genotyping in the veterinary field can be applied in three different areas: (a) purely diagnostic purposes for classification of Campylobacter species and subspecies, (b) typing methods useful for monitoring or surveillance of animals as well as food products of animal origin, and (c) typing methods that can be applied during outbreaks and for source tracing. In addition, typing methods applied in areas (b) and (c) should be distinguished in regard to local short-term and global long-term epidemiology, respectively.
While a whole plethora of discriminative typing methods are available, classification tools of certain species and subspecies are still missing. Perspectively, as the genomes of many relevant Campylobacter species have now been sequenced, this will help to identify several species specific loci, the products of which should be available to develop easy and fast applicable diagnostic tools. Global cooperation, sharing of strains and databases should close the currently existing gaps in Campylobacter identification tools.

• 出版日期2007-8