Melanoma cell adhesion molecule identifies encephalitogenic T lymphocytes and promotes their recruitment to the central nervous system

作者：Larochelle Catherine; Cayrol Romain; Kebir Hania; Alvarez Jorge Ivan; Lecuyer Marc Andre; Ifergan Igal; Viel Emilie; Bourbonniere Lyne; Beauseigle Diane; Terouz Simone; Hachehouche Lamia; Gendron Steve; Poirier Josee; Jobin Celine; Duquette Pierre; Flanagan Ken; Yednock Ted; Arbour Nathalie; Prat Alexandre^*

来源：Brain, 2012, 135(10): 2906-2924.

DOI：10.1093/brain/aws212

• MCAM (CD146)
• multiple sclerosis
• T(H)17
• blood-brain barrier
• EAE
• IL23
• leukocyte transmigration
• adhesion
• diapedesis

In multiple sclerosis, encephalitogenic CD4(+) lymphocytes require adhesion molecules to accumulate into central nervous system inflammatory lesions. Using proteomic techniques, we identified expression of melanoma cell adhesion molecule (MCAM) on a subset of human effector memory CD4(+) lymphocytes and on human blood-brain barrier endothelium. Herein, we demonstrate that MCAM is a stable surface marker that refines the identification of interleukin 17(+), interleukin 22(+), RAR-related orphan receptor gamma and interleukin 23 receptor(+) cells within the CD161(+)CCR6(+) subset of memory CD4(+) lymphocytes. We also show that MCAM(+) lymphocytes express significantly more granulocyte/macrophage colony stimulating factor and granzyme B than MCAM(-) lymphocytes. Furthermore, the proportion of MCAM(+) CD4(+) lymphocytes is significantly increased in the blood and in the central nervous system of patients with multiple sclerosis and experimental autoimmune encephalomyelitis animals compared with healthy controls or other neurological diseases, and MCAM expression is upregulated at the blood-brain barrier within inflammatory lesions. Moreover, blockade of MCAM or depletion of MCAM(+) CD4(+) T lymphocytes both restrict the migration of T(H)17 lymphocytes across blood-brain barrier endothelial cells and decrease the severity of experimental autoimmune encephalomyelitis. Our findings indicate that MCAM could serve as a potential biomarker for multiple sclerosis and represents a valuable target for the treatment of neuroinflammatory conditions.

• 出版日期2012-10