Detecting weak protein-protein interactions by modified far-western blotting

作者：Sato Yuya; Kameya Masafumi; Arai Hiroyuki; Ishii Masaharu^*; Igarashi Yasuo

来源：Journal of Bioscience and Bioengineering, 2011, 112(3): 304-307.

DOI：10.1016/j.jbiosc.2011.05.011

• Far western blotting
• Protein-protein interaction
• Ferredoxin
• Hydrogenobacter thermophilus
• Electron carrier

We developed a cross-linking far-western blotting (WB) technique to detect weak protein-protein interactions. In addition, by comparison of the banding patterns resulting from the conventional and cross-linking methods, weak interactions can be identified by monitoring the enhancement of band intensities.

• 出版日期2011-9