Central Composite Design for Optimized Extraction of Polysaccharides from Undaria pinnatifida

作者：Zhu Tao; Heo Hyo jung; Row Kyung ho^*

来源：Chemical Research in Chinese Universities, 2012, 28(4): 620-623.

• Undaria pinnatifida
• Optimization
• Extraction
• Polysaccharide
• Response surface methodology

Response surface methodology was used to optimize the extraction conditions of polysaccharides from Undaria pinnatifida. The effects of three independent variables; extraction time, extraction temperature and ratio of water to raw material were investigated by a central composite design. The experimental data were fitted to a second-order polynomial equation via multiple regression analysis and also examined with the appropriate statistical methods. The adjusted coefficient of determination(R-Adj(2)) for the model was 0.9171. An optimum extraction yield of 30.78% was obtained when the extraction temperature was 95 degrees C, extraction time was 4.5 h, and the ratio of water to raw material was 35(mL/g). Under these conditions, validation experiments were done and the mean extraction yield of polysaccharides was 30.79%, which was in good agreement with the predicted model value.

• 出版日期2012-7-25