Abelson interacting protein 1 (Abi-1) is essential for dendrite morphogenesis and synapse formation

作者：Proepper Christian; Johannsen Svenja; Liebau Stefan; Dahl Janine; Vaida Bianca; Bockmann Juergen; Kreutz Michael R; Gundelfinger Eckart D; Boeckers Tobias M^*

来源：The EMBO Journal, 2007, 26(5): 1397-1409.

DOI：10.1038/sj.emboj.7601569

• actin
• ProSAP2
• PSD
• Shank3
• synapse

Synaptogenesis and synaptic plasticity depend crucially on the dynamic and locally specific regulation of the actin cytoskeleton. We identified an important component for controlled actin assembly, abelson interacting protein-1 (Abi-1), as a binding partner for the postsynaptic density (PSD) protein ProSAP2/Shank3. During early neuronal development, Abi-1 is localized in neurites and growth cones; at later stages, the protein is enriched in dendritic spines and PSDs, as are components of a trimeric complex consisting of Abi-1, Eps8 and Sos-1. Abi-1 translocates upon NMDA application from PSDs to nuclei. Nuclear entry depends on abelson kinase activity. Abi-1 co-immunoprecipitates with the transcription factor complex of Myc/Max proteins and enhances E-box-regulated gene transcription. Downregulation of Abi-1 by small interfering RNA results in excessive dendrite branching, immature spine and synapse morphology and a reduction of synapses, whereas overexpression of Abi-1 has the opposite effect. Data show that Abi-1 can act as a specific synaptonuclear messenger and is essentially involved in dendrite and synapse formation.

• 出版日期2007-3-7