Nuclear deadenylation/polyadenylation factors regulate 3 ' processing in response to DNA damage

作者：Cevher Murat A; Zhang Xiaokan; Fernandez Sully; Kim Sergey; Baquero Jorge; Nilsson Per; Lee Sean; Virtanen Anders; Kleiman Frida E^*

来源：The EMBO Journal, 2010, 29(10): 1674-1687.

DOI：10.1038/emboj.2010.59

• 3 ' RNA processing
• deadenylation
• DNA damage
• polyadenylation

We previously showed that mRNA 3' end cleavage reaction in cell extracts is strongly but transiently inhibited under DNA-damaging conditions. The cleavage stimulation factor-50 (CstF-50) has a role in this response, providing a link between transcription-coupled RNA processing and DNA repair. In this study, we show that CstF-50 interacts with nuclear poly(A)-specific ribonuclease (PARN) using in vitro and in extracts of UV-exposed cells. The CstF-50/PARN complex formation has a role in the inhibition of 3' cleavage and activation of deadenylation upon DNA damage. Extending these results, we found that the tumour suppressor BARD1, which is involved in the UV-induced inhibition of 3' cleavage, strongly activates deadenylation by PARN in the presence of CstF-50, and that CstF-50/BARD1 can revert the cap-binding protein-80 (CBP80)mediated inhibition of PARN activity. We also provide evidence that PARN along with the CstF/BARD1 complex participates in the regulation of endogenous transcripts under DNA-damaging conditions. We speculate that the interplay between polyadenylation, deadenylation and tumour-suppressor factors might prevent the expression of prematurely terminated messengers, contributing to control of gene expression under different cellular conditions. The EMBO Journal (2010) 29, 1674-1687. doi: 10.1038/emboj.2010.59; Published online 8 April 2010

• 出版日期2010-5-19