A universal transcription pause sequence is an element of initiation factor sigma 70-dependent pausing

作者：Bird Jeremy G; Strobel Eric J; Roberts Jeffrey W^*

来源：Nucleic Acids Research, 2016, 44(14): 6732-6740.

DOI：10.1093/nar/gkw285

The Escherichia coli sigma 70 initiation factor is required for a post-initiation, promoter-proximal pause essential for regulation of lambdoid phage late gene expression; potentially, sigma 70 acts at other sites during transcription elongation as well. The pause is induced by sigma 70 binding to a repeat of the promoter -10 sequence. After sigma 70 binding, further RNA synthesis occurs as DNA is drawn (or 'scrunched') into the enzyme complex, presumably exactly as occurs during initial synthesis from the promoter; this synthesis then pauses at a defined site several nucleotides downstream from the active center position when sigma 70 first engages the -10 sequence repeat. We show that the actual pause site in the stabilized, scrunched complex is the 'elemental pause sequence' recognized from its frequent occurrence in the E. coli genome. sigma 70 binding and the elemental pause sequence together, but neither alone, produce a substantial transcription pause.

• 出版日期2016-8-19
• 单位rutgers