Alterations in the expression of protease-activated receptor 1 and tumor necrosis factor-alpha in the basilar artery of rats following a subarachnoid hemorrhage

作者：Li, Gang^*; Wang, Qing-Song; Lin, Ting-Ting

来源：Experimental and Therapeutic Medicine, 2016, 11(3): 717-722.

DOI：10.3892/etm.2016.3010

• subarachnoid hemorrhage
• cerebral vasospasm
• protease-activated receptor 1
• tumor necrosis factor-alpha

The present study aimed to investigate the expression of protease-activated receptor 1 (PAR1) and tumor necrosis factor (TNF)-alpha in a rat model of subarachnoid hemorrhage (SAH)-induced cerebral vasospasm (CVS). The rat models were established by twice injecting blood into the cisterna magna, after which the following experimental groups were established: The normal group, the SAH3d group, the SAH5d group and the SAH7d group. The rats were perfused and the basilar artery was removed for histological examination. The cross-sectional area of the basilar artery lumen was measured using computer software; and the protein expression of PAR1 and TNF-alpha was detected by immunohistochemistry. The cross-sectional area of the basilar artery of the rats in the SAH model groups was significantly decreased in a time-dependent manner, as compared with the normal group. The protein expression of PAR1 and TNF-alpha in the SAH3d, SAH5d and SAH7d groups was significantly increased over time (P<0.05), as compared with the normal group. CVS was detected in the basilar artery, and was associated with wall thickening and significant narrowing of the lumen, thus suggesting that the present model may be used for investigating cerebrovascular disease following SAH. The immunohistochemical analyses demonstrated that the protein expression of PAR1 and TNF-alpha was significantly increased in the basilar artery of the SAH model rats, and were positively correlated with the degree of CVS.

• 出版日期2016-3
• 单位中国人民解放军总医院; 海口市人民医院