Determination of Antioxidant Capacity Using the Biological System Bacteriophage P22/Bacterium Salmonella typhimurium

作者：Giao Maria S; Borges Andre B; Guedes Catarina I; Hogg Tim A; Pintado Manuela E; Malcata F Xavier^*

来源：Journal of Agricultural and Food Chemistry, 2009, 57(1): 22-25.

DOI：10.1021/jf802185k

• Ascorbic acid
• gallic acid
• in vivo assessment

Bacteriophage/bacterium systems have been employed in the past in assays for virucidal activity. A novel application of one such system is proposed here for the in vivo determination of antioxidant capacity. It was shown that an antioxidant such as gallic acid can effectively protect against oxidative damage brought about by H(2)O(2)-but only within a narrow range of concentrations (i.e., from 250 to 500 mg L(-1)); ascorbic acid, on the other hand, did not exhibit any protective effect against H(2)O(2). Finally, neither ascorbic nor gallic acid demonstrated a virucidal effect. The P22/Salmonella typhimurium model system thus proved to be useful in the assessment of antioxidant capacity in vivo, at least using those two alternative model antioxidants.

• 出版日期2009-1-14