A new enzyme-linked immunosorbent assay system for human hepatic triglyceride lipase

作者：Miyashita Kazuya; Kobayashi Junji^*; Imamura Shigeyuki; Kinoshita Noriaki; Stanhope Kimber L; Havel Peter J; Nakajima Katsuyuki; Machida Tetsuo; Sumino Hiroyuki; Nara Makoto; Murakami Masami

来源：Clinica Chimica Acta, 2013, 424: 201-206.

DOI：10.1016/j.cca.2013.06.016

• Hepatic triglyceride lipase
• Endothelial lipase
• ELISA

Background: The objective of this study was to establish a new sandwich based enzyme linked immunosorbent assay (ELISA) for measuring the protein mass of human hepatic triacylglyceride lipase (HTGL). %26lt;br%26gt;Method: Two mouse monoclonal antibodies raised against human HTGL were used for the sandwich ELISA. The post-heparin plasma (PHP) samples obtained at a heparin dose of 50 unit/kg from 124 normolipidemic subjects were used for this ELISA. %26lt;br%26gt;Results: The dynamic assay range of the developed ELISA for the HTGL was from 0.47 to 30 ng/ml. The CV was %26lt;7% in both intra- and inter-assays, and it did not cross-react with lipoprotein lipase or endothelial lipase (EL). The HTGL concentration in PHP showed a strong correlation with HTGL activity [n = 121, r = 0.778, p %26lt; 0.001]. There was a weak relation of HTGL concentration against high-density lipoprotein cholesterol (HDL-C) [n = 123, r = -0.229, p = 0.011] but no relations against total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), triglycerides (TG), small dense LDL, remnant like particles cholesterol (RLP-C) and RLP-TG were confirmed. Interestingly, a weak but positive correlation between HTGL concentration and EL concentration was shown [n = 122, p = 0.013, r = 0.224]. %26lt;br%26gt;Conclusion: These results indicate that this new sandwich ELISA for measuring HTGL concentration in PHP can be applied in a daily clinical practice.

• 出版日期2013-9-23