Anti-hypertensive activity of genetically modified soybean seeds accumulating novokinin

作者：Yamada Yuko^*; Nishizawa Keito; Yokoo Megumi; Zhao Hui; Onishi Kunihiko; Teraishi Masayoshi; Utsumi Shigeru; Ishimoto Masao; Yoshikawa Masaaki

来源：Peptides, 2008, 29(3): 331-337.

DOI：10.1016/j.peptides.2007.11.018

• novokinin soybean p-conglycinin
• hypotensive activity
• transgenic soybean
• spontaneously hypertensive rat

Novokinin (Arg-Pro-Leu-Lys-Pro-Trp), which has been designed based on the structure of ovokinin (2-7), significantly reduces the systolic blood pressure at a dose of 100 mu g/kg after oral administration in spontaneously hypertensive rats (SHRs). In this study, we generated a transgenic soybean which accumulates novokinin. A vector encoding a modified P-conglycinin alpha' subunit (4novokinin-alpha') in which four novokinin sequences have been incorporated by site-directed mutagenesis was introduced into somatic embryos by whisker-mediated gene transformation to produce a transgenic soybean. The 4novokinin-a' occupied 0.5% of total soluble protein and 5% of the P-conglycinin a' subunit in the transgenic soybean seeds. Protein extracted from the transgenic soybean reduced systolic blood pressure after single oral administration in SHRs at a dose of 0.15 g/kg. Defatted flour from the transgenic soybean also reduced the systolic blood pressure at a dose of 0.25 g/kg. Thus, the 4novokinin-alpha' produced in soybean exhibited an anti -hypertensive activity in SHRs after oral administration.

• 出版日期2008-3