A method to analyze, sort, and retain viability of obligate anaerobic microorganisms from complex microbial communities

作者：Thompson Anne W^*; Crow Matthew J; Wadey Brian; Arens Christina; Turkarslan Serdar; Stolyar Sergey; Elliott Nicholas; Petersen Timothy W; van den Engh Ger; Stahl David A; Baliga Nitin S

来源：Journal of Microbiological Methods, 2015, 117: 74-77.

DOI：10.1016/j.mimet.2015.07.009

• Anaerobic microorganism
• Flow cytometry
• Controlled-environment sorting
• Single cells

A high speed flow cytometric cell sorter was modified to maintain a controlled anaerobic environment. This technology enabled coupling of the precise high-throughput analytical and cell separation capabilities of flow cytometry to the assessment of cell viability of evolved lineages of obligate anaerobic organisms from cocultures.

• 出版日期2015-10