The Xenopus homeobox gene pitx3 impinges upon somitogenesis and laterality

作者：Smoczer Cristine; Hooker Lara; Brode Sarah; Wolanski Marian; KhosrowShahian Farhad; Crawford Michael^*

来源：Biochemistry and Cell Biology, 2013, 91(2): 79-87.

DOI：10.1139/bcb-2012-0057

• Pitx3
• mutant
• morpholino
• somitogenesis
• laterality

Pitx3 has been identified as the causative locus in a developmental eye mutation associated with mammalian anterior segment dysgenesis, congenital cataracts, and aphakia. In recent studies of frog eye development we discovered that pitx3 expresses symmetrically in the somites and lateral plate mesoderm and asymmetrically during cardiac and gut looping. We report that disruption of pitx3 activity on one side of an embryo relative to the other, either by over-or underexpression of pitx3, elicits a crooked dorsal axis in embryos that is a consequence of a retarded progression through somitogenesis. Unlike in amniotes, Xenopus somites form as cohorts of presomitic cells that rotate perpendicular to the dorsal axis. Since no vertebral anomalies have been reported in mouse and human Pitx3 mutants, we attempt to distinguish whether the segmentation clock is uniquely affected in frog or if the pitx3 perturbation inhibits the cellular changes that are necessary to rotation of presomitic cells. In Xenopus, pitx3 appears to inhibit the rotation of presomitic cell cohorts and to be necessary to the bilaterally symmetric expression of pitx2 in somites.

• 出版日期2013-4