Analysis of the minimal specificity of CED-3 using a yeast transcriptional reporter system

作者：Westein Sarah J; Scott Fiona L; Hawkins Christine J^*

来源：Biochimica et Biophysica Acta-Molecular Cell Research, 2008, 1783(3): 448-454.

DOI：10.1016/j.bbamcr.2007.10.003

• caspase
• protease
• S. cerevisiae
• C. elegans
• nematode
• substrate

Determination of the substrate specificity of site-specific proteases helps define their physiological roles. We developed a yeast-based system for defining the minimal substrate specificity of site-specific proteases, within the context of a protein. Using this system, we characterized the P4-P1 substrate specificity of the nematode apoptotic caspase CED-3. Apart from an absolute requirement for aspartate at the P I position, CED-3 is a relatively promiscuous caspase capable of cleaving substrates bearing many amino acids at P4-P2 sites.

• 出版日期2008-3
• 单位河北医科大学