A New Catechol Biosensor Immobilized Polyphenol Oxidase by Combining Electropolymerization and Cross-Linking Process

作者：Chen, Hang; Li, Shengqi; Wang, Shangde; Tan, Yongyan; Kan, Jinqing^*

来源：International Journal of Polymeric Materials and Polymeric Biomaterials, 2013, 62(12): 620-626.

DOI：10.1080/00914037.2012.761629

• Catechol
• cross-linking process
• one-step process
• polymer synthesis
• polyphenol oxidase

A highly stable and effective catechol biosensor was prepared by immobilizing polyphenol oxidase (PPO) into polyaniline (PANI) film by using the direct electropolymerization (one-step) process in conjunction with cross-linking with glutaraldehyde. The results of electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) confirmed that the immobilization of PPO was successful. The biosensor has a fast response to catechol (10s) with a linear range of 1.0100 mu M and a detection limit of 0.01 mu M. The maximum response current (I-max) and the Michaelis-Menten constant (k'(m)) were found to be 7.94 mu A and 81.32 mu M, respectively. The activation energy (E-a) of the PPO catalytic reaction was 16.5kJ/mol. The biosensor exhibited good stability by retaining 80% of its original activity when stored in a dry state at 4 degrees C for up to 5 months.

• 出版日期2013-6-18
• 单位江苏农牧科技职业学院; 扬州大学