Potentiating activity of luteolin on membrane permeabilizing agent and ATPase inhibitor against methicillin-resistant Staphylococcus aureus

作者：Joung Dae Ki; Lee Young Seob; Han Sin Hee; Lee Sang Won; Cha Seon Woo; Mun Su Hyun; Kong Ryong; Kang Ok Hwa; Song Ho Jun; Shin Dong Won; Kwon Dong Yeul^*

来源：Asian Pacific Journal of Tropical Medicine, 2016, 9(1): 16-19.

DOI：10.1016/j.apjtm.2015.12.004

• Iuteolin
• Methicillin-resistant Staphylococcus aureus
• Membrane permeabilizing agent
• ATPase inhibitor
• Peptidoglycan

Objective: To investigate the mechanism of antibacterial activity of luteolin (LUT) against methicillin-resistant Staphylococcus aureus (MRSA). Methods: The mechanism of anti-MRSA activity of LUT was analyzed by the viability assay in membrane permeabilizing agent, ATPase inhibitors, and peptidoglycan (PGN) derived from Staphylococcus aureus (S. aureus). Also, transmission electron microscopy was used to monitor survival characteristics and changes in S. aureus morphology. Results: Compared to the LUT alone, the optical density of suspensions treated with the combination of 125 mu g/mL Tris and 250 mu g/mL DCCD were reduced to 60% and 46%, respectively. PGN (15.6 mu g/mL) gradually impeded the activity of LUT, and PUN (62.5 mu g/mL) completely blocked the activity of LUT on S. aureus. Conclusions: Increased susceptibility to LUT with the Tris and DCCD combinations is evident in all tested MRSA isolates. The results indicate LUT synergy in increasing cytoplasmic membrane permeability and inhibiting ATPase. S. aureus PGN directly blocks the antibacterial activity of LUT, suggesting the direct binding of LUT with PUN. These findings may be validated for the development of antibacterial agent for low MRSA resistance.

• 出版日期2016-1