Use of Methylmalonyl-CoA Epimerase in Enhancing Crotonase Stereoselectivity

作者:Hamed Refaat B; Gomez Castellanos J Ruben; Froese D Sean; Krysztofinska Ewelina; Yue Wyatt W; Schofield Christopher J*
来源:ChemBioChem, 2016, 17(6): 471-473.
DOI:10.1002/cbic.201500644

摘要

The use of methylmalonyl-CoA epimerase (MCEE) to improve stereoselectivity in crotonase-mediated biocatalysis is exemplified by the coupling of MCEE, crotonyl-CoA carboxylase reductase and carboxymethylproline synthase in a three-enzyme one-pot sequential synthesis of functionalised C-5 carboxyalkylprolines starting from crotonyl-CoA and carbon dioxide.

  • 出版日期2016-3-15