Toll-like receptor 7 and 9 expression in peripheral blood mononuclear cells from patients with chronic hepatitis B and related hepatocellular carcinoma

作者：Xu Ning; Yao Hang ping; Sun Zhen; Chen Zhi^*

来源：Acta Pharmacologica Sinica, 2008, 29(2): 239-244.

DOI：10.1111/j.1745-7254.2008.00711.x

• hepatocellular carcinoma
• peripheral blood mononuclear cells
• hepatitis B
• innate immunity
• receptors
• viruses
• Toll-like receptor 7
• Toll-like receptor 9

Aim: The aim of the present study was to investigate the expression of Toll-like receptors (TLR) 7 and 9 in peripheral blood mononuclear cells (PBMC) of patients with chronic hepatitis B virus (HBV) infection and related hepatocellular carcinoma. Methods: The study group was comprised of 52 patients: 41 with chronic hepatitis B and 11 healthy controls. The protein and mRNA levels of TLR7 and TLR9 were evaluated using real-time PCR, Western blot analysis, and flow cytometry. We also detected the serum viral load of HBV in the patients and analyzed the correlation between HBV-DNA copies and the TLR expression. Results: Our results demonstrated a lower TLR7 expression in all HBV infection groups compared to the controls. We found that HBV infection led to a decreased expression of TLR9 mRNA, but an increased expression of the TLR9 protein compared to the healthy group. The TLR protein levels are related to serum HBV-DNA (P < 0.01). Conclusion: There are downregulations of TLR7 expression and TLR9 mRNA in PBMC of HBV-infected patients, but an increased TLR9 expression at the protein level.

• 出版日期2008-2
• 单位浙江大学; 河北医科大学