Dipeptidyl Peptidase IV-Inhibitory Peptides Derived from Silver Carp (Hypophthalmichthys molitrix Val.) Proteins

作者：Zhang, Ying; Chen, Ran; Chen, Xiling; Zeng, Zhu; Ma, Huiqin; Chen, Shangwu^*

来源：Journal of Agricultural and Food Chemistry, 2016, 64(4): 831-839.

DOI：10.1021/acs.jafc.5b05429

• dipeptidyl peptidase IV
• silver carp protein
• peptide inhibitor
• in silico analysis
• Pro-motif peptide
• LC-MS/MS identification

The dipeptidyl peptidase N (DPP-IV)inhibitory bioactivity of silver carp protein (SCP) hydrolysates were investigated, and their containing efficacious DPP-IV-inhibitory peptides were explored by in silico hydrolysis analysis, peptide separation combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) identification, and chemical synthesis. SCP hydrolysates generated by six proteases all showed efficient DPP-IV-inhibitory activities, and Neutrase-generated hydrolysates had the greatest DPP-IV inhibition (IC50 of 1.12 mg/mL). In silico Neutrase hydrolysis revealed hundreds of fragments released from myosin, actin, and collagen of SCPs, which include different Pro-motif peptides but only three reported peptidic DPP-IV inhibitors with moderate or weak bioactivity, In addition, three new DPP-IV-inhibitory peptides were identified using LC-MS/MS; in particular, LPHDI and APGPAGP showed high DPP-IV-inhibitory activity with IC50 of 105.44 and 229.14 mu M, respectively, and behaved in competitive/non-competitive mixed-type DPP-IV inhibition mode. The results indicate that the SCP-derived DPP-IV-inhibitory peptides could be potential functional ingredients in the diabetic diet.

• 出版日期2016-2-3
• 单位中国农业大学