High glucose induces rat mesangial cells proliferation and MCP-1 expression via ROS-mediated activation of NF-κB pathway, which is inhibited by eleutheroside E

作者：Yang, Xiuqin; Wang, Yangang; Gao, Guanqi^*

来源：Journal of Receptors and Signal Transduction, 2016, 36(2): 152-157.

DOI：10.3109/10799893.2015.1061002

• Cell proliferation
• eleutheroside E
• high glucose
• mesangial cells
• monocyte chemoattractant protein-1
• nuclear factor kappa B
• reactive oxygen species

Glomerular hypertrophy and extracellular matrix accumulation are early features of diabetic nephropathy (DN). High glucose-induced oxidative stress is implicated in the etiology of DN. This study aims to investigate the effect of eleutheroside E (EE) on high glucose mediated rat mesangial cells (MCs) proliferation and monocyte chemoattractant protein-1 (MCP-1) expression and the underlying mechanism. MCs proliferation was assessed by MTT assay. Reactive oxygen species (ROS) level and MCP-1 expression were evaluated by ELISA kit. The protein expression of p47, NF-B p65, p-NF-B p65, IB, p-IB, IKK and p-IKK were determined by Western blot. The results showed that treatment with EE markedly attenuated high glucose induced MCs proliferation and in a dose-dependent manner. Intervention with EE also significantly blocked high glucose induced intracellular ROS production by decreasing NADPH oxidase activity. Meanwhile, EE administration could effectively alleviate the high glucose-stimulated activation of NF-B, the degradation of IB and the expression of MCP-1. These results demonstrate that high glucose enhances MCs proliferation and MCP-1 expression by activating the ROS/NF-B pathway and can be inhibited by EE. Our findings provide a new perspective for the clinical treatment of DN.

• 出版日期2016-3-3
• 单位临沂市人民医院; 青岛大学