A Hydrophobic Small Protein, BpOF4_01690, Is Critical for Alkaliphily of Alkaliphilic Bacillus pseudofirmus OF4

作者：Takahashi Tetsuaki; Krulwich Terry A; Ito Masahiro^*

来源：Frontiers in Microbiology, 2018, 9: 1994.

DOI：10.3389/fmicb.2018.01994

• alkaliphiles
• small protein
• Bacillus pseudofirmus
• respiratory chain
• pH homeostasis
• alkaliphily

A monocistronic small protein, BpOF4_01690, was annotated in alkaliphilic Bacillus pseudofirmus OF4. It comprises 59 amino acids and is hydrophobic. Importantly, homologs of this protein were identified only in alkaliphiles. In this study, a mutant with a BpOF4_01690 gene deletion (designated Delta 01690) exhibited weaker growth than that of the wild type in both malate-based defined and glucose-based defined media under low-sodium conditions at pH 10.5. Additionally, the enzymatic activity of the respiratory chain of Delta 01690 was much lower than that of the wild type. These phenotypes were similar to those of a ctaD deletion mutant and an atpB-F deletion mutant. Therefore, we hypothesize that BpOF4_01690 plays a critical role in oxidative phosphorylation under highly alkaline conditions.

• 出版日期2018-8-28