Dispase rapidly and effectively purifies Schwann cells from newborn mice and adult rats

作者：Zhu, Jiaxue; Qin, Jinbao; Shen, Zunli^*; Kretlow, James D; Wang, Xiaopan; Liu, Zhangyin; Jin, Yuqing

来源：Neural Regeneration Research, 2012, 7(4): 256-260.

DOI：10.3969/j.issn.1673-5374.2012.04.003

• dispase
• differential detachment
• purification
• Schwann cell
• neural regeneration

In the present study, Schwann cells were isolated from the sciatic nerve of neonatal mice and purified using dispase and collagenase. Results showed that after the first round of purification with dispase, most of the Schwann cells appeared round in shape and floated in culture solution after 15 minutes. In addition, cell yield and cell purity were higher when compared to the collagenase group. After the second round of purification, the final cell yield for the dispase group was higher than that for the collagenase group, but no significant difference was found in cell purity. Moreover, similar results in cell quantity and purity were observed in adult Sprague-Dawley rats. These findings indicate that purification with dispase can result in the rapid isolation of Schwann cells with a high yield and purity.

• 出版日期2012-2
• 单位上海交通大学