DIMINISHED PREVALENCE BUT PRESERVED SYNAPTIC DISTRIBUTION OF N-METHYL-D-ASPARTATE RECEPTOR SUBUNITS IN THE METHYL CpG BINDING PROTEIN 2(MeCP2)-NULL MOUSE BRAIN

作者：Maliszewska Cyna E; Bawa D; Eubanks J H^*

来源：Neuroscience, 2010, 168(3): 624-632.

DOI：10.1016/j.neuroscience.2010.03.065

• brain
• autism-spectrum disorder
• methyl DNA-binding factor
• lipid raft
• post-synaptic density

In this study, we examined the prevalence and distribution of NMDA receptor subunits within crude synaptic membranes derived from the brains of mice lacking methyl CpG binding protein 2 (MeCP2). Our results show that the distribution of NMDA receptor subunits within the detergent soluble, detergent resistant, and postsynaptic density microdomains is preserved at MeCP2-null synapses. However, analysis of the NMDA receptor subunit expression revealed a decrease in the prevalence of the GluN1 and GluN2A subunits in MeCP2-null tissue. Collectively, these results indicate that synaptic membrane microdomains at synapses of the MeCP2-null brain develop normally, and that NMDA receptor subunits are properly targeted and distributed within them. The under-representation of the GluN1 and GluN2A subunits suggests that MeCP2-null synapses contain fewer mature NMDA receptor complexes, and raises the possibility that impaired NMDA receptor ontogeny could contribute to Rett syndrome pathophysiology.

• 出版日期2010-7-14
• 单位河北医科大学