Activation of the Pacidamycin PacL Adenylation Domain by MbtH-like Proteins

作者：Zhang Wenjun; Heemstra John R Jr; Walsh Christopher T^*; Imker Heidi J

来源：Biochemistry, 2010, 49(46): 9946-9947.

DOI：10.1021/bi101539b

Nonribosomal peptide synthetase (NRPS) assembly lines are major avenues for the biosynthesis of a vast array of peptidyl natural products. Several hundred bacterial NRPS gene clusters contain a small (similar to 70-residue) protein belonging to the MbtH family for which no function has been defined. Here we show that two strictly conserved Trp residues in MbtH-like proteins contribute to stimulation of amino acid adenylation in some NRPS modules. We also demonstrate that adenylation can be stimulated not only by cognate MbtH-like proteins but also by homologues from disparate natural product pathways.

• 出版日期2010-11-23