A novel recombinant Salmonella vaccine enhances the innate immunity of NK cells against acute myeloid leukaemia cells Kasumi-1 in vitro

作者：Zhang, Bei; Liu, Yuan; Wang, Xiuju; Jiang, Ling; Lu, Zhigang; Zhang, Xing; Li, Kunpeng^*; Hu, Haiyan

来源：Cell Biology International, 2013, 37(12): 1320-1329.

DOI：10.1002/cbin.10173

• DNA vaccine
• innate immunity
• NK cells
• Salmonella

Minor histocompatibility antigen HA-1-specific cytotoxic lymphocyte (CTL) clones have apparent anti-leukaemic efficacy, and the AML/ETO gene is a special fusion gene in leukaemic cells. Thus, we hypothesised that a vaccine targeting HA-1 and AML/ETO could stimulate NK cells to target leukaemia cells. Furthermore, we packaged the vaccine using attenuated Salmonella to enhance its immuno-activity. Expression of the NK cell-activating ligand ULBP2 was notably elevated upon packaging in a co-recombinant group. An AML/ETO single plasmid gave the weakest vaccine. The level of miR-182, which targets ULBP2, significantly decreased with increasing IFN- and granzyme B in a co-recombinant group. In summary, DNA vaccines including AML/ETO and HA-1 fragments significantly enhance the innate immunity of NK cells in vitro.

• 出版日期2013-12
• 单位南方医科大学; 上海交通大学; 中山大学