Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections

作者：Mlodzianoski Michael J; Cheng Hathaway Paul J; Bemiller Shane M; McCray Tyler J; Liu Sheng; Miller David A; Lamb Bruce T; Landreth Gary E; Huang Fang^*

来源：Nature Methods, 2018, 15(8): 583-+.

DOI：10.1038/s41592-018-0053-8

Application of single-molecule switching nanoscopy (SMSN) beyond the coverslip surface poses substantial challenges due to sample-induced aberrations that distort and blur single-molecule emission patterns. We combined active shaping of point spread functions and efficient adaptive optics to enable robust 3D-SMSN imaging within tissues. This development allowed us to image through 30-mu m-thick brain sections to visualize and reconstruct the morphology and the nanoscale details of amyloid-beta filaments in a mouse model of Alzheimer's disease.

• 出版日期2018-8